How does digital sampling affect resolution
Look at imaging these object with a digital camera
How close together do pixels need to be?
Sampling over time:
How often do you need to image a moving sample
Nyquist theory states that you should sample more than 2 X the frequency that you expect.
Under sampling causes aliasing
Optical Transfer Function MTF
When objects get close together the contrast decreases.
MTF = Image Modulation/Object Modulation
MTF = 2(φ - cosφsinφ)/π and
φ = cos-1(λν/2NA)
The Optical Transfer function is the Modulation transfer function times a phase component.
OTF = MTF × eiφ(f)
Camera bit depth
0 or 1
00 or 01 or 10 or 11
000 or 001 or 010 or 011 or 100 or 101 or 110 or 111
and so on
Jpg is 8 bit Tiff can be 16 bit