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IGB Core

Information on the IGB core

IGB Core Training

This book will contain Training materials for each instrument

BookitLab Guide

User Guides for basic uses of BookitLab

Workshops

These are Workshops hosted by the IGB Core

Miscellaneous Software

Guides for various pieces of software

LSM 710

IGB Core Training

Basic Optics

IGB Core Training

Initial Training for Optical Microscope Users

600MHz NMR

IGB Core Training

NMR Training

Lsm 700 Info

IGB Core Training

Nanozoomer

IGB Core Training

Cryostat

IGB Core Training

MINFLUX

IGB Core Training

Molecular resolution in 2D and 3D

Reserve Instrument Time

BookitLab Guide

Initiate Usage (Cryostat/Microtome/NMR)

BookitLab Guide

Purchase a Consumable

BookitLab Guide

Logging in to your reservation

BookitLab Guide

Accessing the Core-Server

BookitLab Guide

Fiji Workshop

Workshops

December 11th 2024

Optics

IGB Core Training Basic Optics

Properties of light Wave particle duality: Light is a wave Absorption and Emission Beer's law Refraction Ray tracing   Resolution Resolution: The ability to separate two objects   A definition of Numerical Aperture   Fourier transform: Tr...

Data Storage

IGB Core Training Basic Optics

Store data on the data drive on the local machine: D\Data may be deleted at anytime.           Do not leave your data here!    The core staff will clean the computer drives occasionally. This means that your profile, desktop and data from the data drive wil...

IGB Core Instruments Axiovert 200M

IGB Core Training Basic Optics

Axiovert 200M Cameras cMOS          

Sampling

IGB Core Training Basic Optics

How does digital sampling affect resolution Look at imaging these object with a digital camera How close together do pixels need to be? Sampling over time: How often do you need to image a moving sample   Nyquist theory states that you should sample ...

Objectives

IGB Core Training Basic Optics

LSM 710 Basic Operation

IGB Core Training LSM 710

Turning On the System Make sure the main power switch, systems switch and components switch are all on. The key should be turned on, please do not touch the key. Turn on the X-Cite lamp next to the microscope. Turn on the Argon ion laser. Turn the key to t...

NMR Theory

IGB Core Training 600MHz NMR

Agilent Hardware

IGB Core Training 600MHz NMR

Basic Operation

IGB Core Training 600MHz NMR

600 MHz NMR “In the absence of a magnetic field the period of all these oscillations is the same. But as soon as the electron is exposed to the effect of a magnetic field, its motion changes.” – Zeeman, P. FIRST PROCEDURES Content:1. Sample preparation2. Lo...

LSM 700 Basic Operation

IGB Core Training Lsm 700 Info

  Startup Turn on the laser lamp Login into the computer Start [ZEN] software - Start System   Visual Operation   Loading a slide (#1.5 cover slides are the best) Set the objective to 10x using the touch pad on the microscope (recommended to alw...

IGB Core Instruments Zeiss LSM 710

IGB Core Training Basic Optics

Light Path https://www.gu.se/en/core-facilities/lsm-710-nlo Seven visible excitation lines: 405nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm. Tisaphire laser 700nm to 980nm Spectral Unmixing http://zeiss-campus.magnet.fsu.edu/articles/spectralimaging/...

Fluorescence Imaging

IGB Core Training Basic Optics

Why Fluorescence: We can label what we want to see   Excitation and Emission Dapi           Widefield vs Confocal       https://www.journals.uchicago.edu/doi/full/10.1086/689588      

IGB Core Instruments LSM 700

IGB Core Training Basic Optics

LSM 700   http://zeiss-campus.magnet.fsu.edu/tutorials/spectralimaging/lsm700/indexflash.html

IGB Core Instrument LSM 880

IGB Core Training Basic Optics

   

IGB Core Instrument V16

IGB Core Training Basic Optics

X5000

IGB Core Training

Nanozoomer Basic Operation

IGB Core Training Nanozoomer

Warning Before beginning, have one of the core staff (listed here) look at the slides to confirm that there are no protruding coverslip edges.  The Nanozoomer is very particular about slide size, any non-standard slides or protruding coverslips will cause it ...

video

IGB Core Training Cryostat

Working in the IGB Core

IGB Core Training Basic Optics

Expect to walk into a room with a fully functional instrument Let a core staff person know if you see a problem Clean up when you leave Acknowledge the IGB Core as:          “Core Facilities at the Carl R. Woese Institute for Genomic Biology" Let us know ...

Types of Labelling for Microscopy

IGB Core Training

The types of labelling used depends on the type of microscope or imaging technique. 1. Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM), Serial Block Face-SEM, etc. requires using heavy metals such as Osmium to label tissues. 2. Lig...