IGB Core Training
This book will contain Training materials for each instrument
600MHz NMR
NMR Training
Axio Scan Z1
Axio Scan Z1 Operating Manual 1. Make sure the slide scanner switch on the back and on the front...
Axio Zoom V16 Operating Manual
Power ON Routine 1. Turn on X-Cite epi-fluorescence light source for fluorescence imaging 2. T...
Basic Optics
Initial Training for Optical Microscope Users
Data Storage
Store data on the data drive on the local machine: D\Data may be deleted at anytime. D...
Fluorescence Imaging
Why Fluorescence: We can label what we want to see Excitation and Emission Dapi ...
IGB Core Instrument LSM 880
IGB Core Instrument LSM 900
405nm, 488nm, 561nm, 640nm excitation. Zen Blue file:///C:/Users/gfried/Downloads/EN_poste...
IGB Core Instrument V16
IGB Core Instruments Axiovert 200M
Axiovert 200M Cameras cMOS
IGB Core Instruments LSM 700
LSM 700 http://zeiss-campus.magnet.fsu.edu/tutorials/spectralimaging/lsm700/indexflash.html
IGB Core Instruments Zeiss LSM 710
Light Path https://www.gu.se/en/core-facilities/lsm-710-nlo Seven visible excitation line...
Objectives
Optics
Properties of light Wave particle duality: Light is a wave Absorption and Emission Beer's...
Sampling
How does digital sampling affect resolution Look at imaging these object with a digital camera ...
Working in the IGB Core
Expect to walk into a room with a fully functional instrument Let a core staff person know if yo...
Cryostat
EMPANADA Guide
To begin, I would recommend that one starts with the official empanada guidebooks as located here...
Köhler Illumination
Köhler Illumination for Brightfield Köhler illumination is a method for evenly illuminating the...
Lsm 700 Info
LSM 710
LSM 900
MINFLUX
Molecular resolution in 2D and 3D
Introduction
Minflux is a florescence imaging and tracking technique using a 2D doughnut or 3D bottle beam to ...
Separating two signal based on dcr (detector channel ratio) in paraview
Goal: to separate signal form 2 detectors (CY5 near vs CY5 far), especially when you have two d...
Superimpose images in Imspector
Goal: Superimpose confocal images Superimpose confocal image with Minflux image Note: Images c...
Tracking in ParaView
Goal: how to analysis tracking data in paraview 1 Imspector: open your image in Imspector 2 M...
Nanozoomer
Types of Labelling for Microscopy
The types of labelling used depends on the type of microscope or imaging technique. 1. Transmiss...