IGB Core Training
This book will contain Training materials for each instrument
Initial Training for Optical Microscope Users
Properties of light Wave particle duality: Light is a wave Absorption and Emission Beer's...
How does digital sampling affect resolution Look at imaging these object with a digital camera ...
Why Fluorescence: We can label what we want to see Excitation and Emission Dapi ...
IGB Core Instruments Axiovert 200M
Axiovert 200M Cameras cMOS
IGB Core Instruments LSM 700
LSM 700 http://zeiss-campus.magnet.fsu.edu/tutorials/spectralimaging/lsm700/indexflash.html
IGB Core Instruments Zeiss LSM 710
Light Path https://www.gu.se/en/core-facilities/lsm-710-nlo Seven visible excitation line...
IGB Core Instrument LSM 880
IGB Core Instrument V16
Store data on the data drive on the local machine: D\Data may be deleted at anytime. D...
Working in the IGB Core
Expect to walk into a room with a fully functional instrument Let a core staff person know if yo...
Lsm 700 Info
Types of Labelling for Microscopy
The types of labelling used depends on the type of microscope or imaging technique. 1. Transmiss...
Axio Scan Z1
Axio Scan Z1 Operating Manual 1. Make sure the slide scanner switch on the back and on the front...
Köhler Illumination for Brightfield Köhler illumination is a method for evenly illuminating the...
Axio Zoom V16 Operating Manual
Power ON Routine 1. Turn on X-Cite epi-fluorescence light source for fluorescence imaging 2. T...
Molecular resolution in 2D and 3D
Minflux is a florescence imaging and tracking technique using a 2D doughnut or 3D bottle beam to ...
Superimpose images in Imspector
Goal: Superimpose confocal images Superimpose confocal image with Minflux image Note: Images c...
Separating two signal based on dcr (detector channel ratio) in paraview
Goal: to separate signal form 2 detectors (CY5 near vs CY5 far), especially when you have two d...
Tracking in ParaView
Goal: how to analysis tracking data in paraview 1 Imspector: open your image in Imspector 2 M...