# Optics

## Properties of light

### Wave particle duality:

Light is a wave Beer's law

Refraction

## Resolution

### Resolution: The ability to separate two objects ### A definition of Numerical Aperture #### Fourier transform:

Transform from real space to frequency space Now we look at a real square wave and frequency space ### Look at a simple optical system: #### Mathematical prediction of the Point Spread Function (PSF)

on the left we have the mathematical point source know as a delta function. were the intensity at the Fourier plain can be found by taking the Fourier transform of this function. or this has the same intensity at all points inside the aperture and zero outside. The second lens is now taking a Fourier transform on a box function the width of the aperture. or Substituting in the definition for NA    ### Now we go back to Resolution. How close together we can position two points and still distinguish them         More intuitive approach Notes from:

http://web.mit.edu/2.710/Fall06/2.710-wk12-b-sl.pdf