Microscopy Effects on Images
Detectors:
Array Detectors
Cameras, CCD's EMCCD's Back thinned CCD's, CMOS, SCMOS
These detectors measure photons and produce electrons in each pixel. The pixels are then readout as a serial list of values representing the photon intensity at each location.
Quantum Efficiency
The quantum efficiency is a ratio between the number of photons hitting a pixel to the number of electrons in the well
|
PCO edge 5.5 sCOMS |
Kinetix sCOMS |
| IXon CCD |
Noise
- Read Noise:
- Errors in reading elections in wells. Read noise is independent of ingratiation time and effects low light level imaging.
- Shot noise:
- statistical differences in the number of photons hitting the pixel
- Dark noise:
- Is the accumulation of electrons when no photons are hitting the pixel
- Hot Pixels/ Dead Pixels:
- Pixels with Higher or lower dark noise
Pixel Size
Use magnification and Nyquist theorem
Point Detectors
PMT's APD's
Quantum Efficiency
Noise
other Info
more stuff
even more stuff
Point Spread Function
A microscope is a 4f system as shown
The objective is the first lens and col
Objectives
https://www.microscopyu.com/digital-imaging/introduction-to-charge-coupled-devices-ccds