Microscopy Effects on Images
Detectors:
Array detectors:
Cameras, CCD's EMCCD's Back thinned CCD's, CMOS, SCMOS
These detectors measure photons and produce electrons in each pixel. The pixels are then readout as a serial list of values representing the photon intensity at each location.
Quantum Efficiency
The quantum efficiency is a ratio between the number of photons hitting a pixel to the number of electrons in the well
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Kinetix sCOMS |
| IXon CCD |
Read Noise:
Errors in reading elections in wells. Read noise is independent of ingratiation time and effects low light level imaging.
Shot noise:
statistical differences in the number of photons hitting the pixel
Dark noise:
Is the accumulation of electrons when no photons are hitting the pixel
Hot Pixels/ Dead Pixels:
Pixels with Higher or lower dark noise
Pixel Size:
Use magnification and Nyquist theorem
Point Spread Function and Blur:
A microscope is a 4f system as shown
Lens 1
https://www.microscopyu.com/digital-imaging/introduction-to-charge-coupled-devices-ccds