Microscopy Effects on Images

Detectors: 

Array detectors:

Cameras, CCD's EMCCD's Back thinned CCD's, CMOS, SCMOS 

These detectors measure photons and produce electrons in each pixel. The pixels are then readout as a serial list of values representing the photon intensity at each location.

 

Quantum Efficiency

The quantum efficiency is a ratio between the number of photons hitting a pixel to the number of electrons in the well

 

 

 

 

 

 

 

 

https://www.microscopyu.com/digital-imaging/introduction-to-charge-coupled-devices-ccds