IGB Core Instruments Zeiss LSM 710

Light Path

https://www.gu.se/en/core-facilities/lsm-710-nlo

Seven visible excitation lines: 405nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm.

Tisaphire laser 700nm to 980nm

 

 

Two Photon: Image Deeper with Less Photo Damage

Two photon uses two photons to excite a single electronic transition. this means that both photons need to be absorbed simultaneously.  Single photon absorption follows Beers law

https://en.wikipedia.org/wiki/Two-photon_absorption#Absorption_rate

Fluorescence Lifetime Imaging Microscopy (FLIM): Separate fluorophores based on average time in the excited state.

start with single photon counting and decay curves

 

then introduce Phasor plots