Instrumentation

IGB Core Instruments

LSM 700

 

http://zeiss-campus.magnet.fsu.edu/tutorials/spectralimaging/lsm700/indexflash.html

LSM 710

 

https://www.gu.se/en/core-facilities/lsm-710-nlo

Two Photon: Image Deeper with Less Photo Damage

Two photon uses two photons to excite a single electronic transition. this means that both photons need to be absorbed simultaneously.  Single photon absorption follows Beers law

https://en.wikipedia.org/wiki/Two-photon_absorption#Absorption_rate

Fluorescence Lifetime Imaging Microscopy:Microscopy (FLIM): Separate fluorophores based on average time in the excited state.

start with single photon counting and decay curves

 

then introduce Phasor plots

 

LSM 880

 

 

 

Elyra

Light sheet