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Axio Scan Z1 Operating Manual 1. Make sure the slide scanner switch on the back and on the front 2. Login to the computer with your NETID and IGB password 3. Start ZEN blue then clicks on ZEN slidescan 4. Load your slides into the trays, place your slides ...

Köhler Illumination for Brightfield Köhler illumination is a method for evenly illuminating the sample with white light. This method is very important for brightfield imaging in widefield microscopy, and for using the T-PMT for confocal imaging. Brightfield ...

Power ON Routine 1. Turn on X-Cite epi-fluorescence light source for fluorescence imaging 2. Turn on EMS3 box 3. On the SYCOP 3, the system will prompt you to calibrate the stage: click yes to perform stage calibration. Make sure there is nothing blockin...

Minflux is a florescence imaging and tracking technique using a 2D doughnut or 3D bottle beam to localize switchable fluorophores to single digit nanometer resolution. Expanding on this Minflux turns on fluorophores using a 405 laser. This allows the microsc...

Goal: Superimpose confocal images Superimpose confocal image with Minflux image Note: Images can be combined if they have the same length and the same pixel size. In general, the pixel size of the Minflux image usually is much bigger than confocal image. As...

Goal: to separate signal form 2 detectors (CY5 near vs CY5 far), especially when you have two dyes for example AF640 and AF680 1. Open image in Imspector 2. Minflux Data Panel → Data source: it is usually None, so you must select data source that you want ...

Goal: how to analysis tracking data in paraview 1 Imspector: open your image in Imspector 2 Minflux Data panel → select data set on Data source panel → Compile trace data (Trace data compiled on the bottom of Minflux Data panel) → then Launch Paraview     ...