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Optics
Properties of light Wave particle duality: Light is a wave Absorption and Emission Beer's law Refraction Ray tracing Resolution Resolution: The ability to separate two objects A definition of Numerical Aperture Fourier transform: Tr...
Data Storage
Store data on the data drive on the local machine: D\Data may be deleted at anytime. Do not leave your data here! The core staff will clean the computer drives occasionally. This means that your profile, desktop and data from the data drive wil...
IGB Core Instruments Axiovert 200M
Axiovert 200M Cameras cMOS
Sampling
How does digital sampling affect resolution Look at imaging these object with a digital camera How close together do pixels need to be? Sampling over time: How often do you need to image a moving sample Nyquist theory states that you should sample ...
Objectives
NMR Theory
Agilent Hardware
IGB Core Instruments Zeiss LSM 710
Light Path https://www.gu.se/en/core-facilities/lsm-710-nlo Seven visible excitation lines: 405nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm. Tisaphire laser 700nm to 980nm Spectral Unmixing http://zeiss-campus.magnet.fsu.edu/articles/spectralimaging/...
Fluorescence Imaging
Why Fluorescence: We can label what we want to see Excitation and Emission Dapi Widefield vs Confocal https://www.journals.uchicago.edu/doi/full/10.1086/689588
IGB Core Instruments LSM 700
LSM 700 http://zeiss-campus.magnet.fsu.edu/tutorials/spectralimaging/lsm700/indexflash.html
IGB Core Instrument LSM 880
IGB Core Instrument V16
X5000
video
Working in the IGB Core
Expect to walk into a room with a fully functional instrument Let a core staff person know if you see a problem Clean up when you leave Acknowledge the IGB Core as: “Core Facilities at the Carl R. Woese Institute for Genomic Biology" Let us know ...
IGB Core Instrument LSM 900
405nm, 488nm, 561nm, 640nm excitation. Zen Blue file:///C:/Users/gfried/Downloads/EN_poster_Beampath-LSM-900_A1.pdf