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16 total results found

Optics

IGB Core Training Basic Optics

Properties of light Wave particle duality: Light is a wave Absorption and Emission Beer's law Refraction Ray tracing   Resolution Resolution: The ability to separate two objects   A definition of Numerical Aperture   Fourier transform: Tr...

Data Storage

IGB Core Training Basic Optics

Store data on the data drive on the local machine: D\Data may be deleted at anytime.           Do not leave your data here!    The core staff will clean the computer drives occasionally. This means that your profile, desktop and data from the data drive wil...

IGB Core Instruments Axiovert 200M

IGB Core Training Basic Optics

Axiovert 200M Cameras cMOS          

Sampling

IGB Core Training Basic Optics

How does digital sampling affect resolution Look at imaging these object with a digital camera How close together do pixels need to be? Sampling over time: How often do you need to image a moving sample   Nyquist theory states that you should sample ...

Objectives

IGB Core Training Basic Optics

NMR Theory

IGB Core Training 600MHz NMR

Agilent Hardware

IGB Core Training 600MHz NMR

IGB Core Instruments Zeiss LSM 710

IGB Core Training Basic Optics

Light Path https://www.gu.se/en/core-facilities/lsm-710-nlo Seven visible excitation lines: 405nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm. Tisaphire laser 700nm to 980nm Spectral Unmixing http://zeiss-campus.magnet.fsu.edu/articles/spectralimaging/...

Fluorescence Imaging

IGB Core Training Basic Optics

Why Fluorescence: We can label what we want to see   Excitation and Emission Dapi           Widefield vs Confocal       https://www.journals.uchicago.edu/doi/full/10.1086/689588      

IGB Core Instruments LSM 700

IGB Core Training Basic Optics

LSM 700   http://zeiss-campus.magnet.fsu.edu/tutorials/spectralimaging/lsm700/indexflash.html

IGB Core Instrument LSM 880

IGB Core Training Basic Optics

   

IGB Core Instrument V16

IGB Core Training Basic Optics

X5000

IGB Core Training

video

IGB Core Training Cryostat

Working in the IGB Core

IGB Core Training Basic Optics

Expect to walk into a room with a fully functional instrument Let a core staff person know if you see a problem Clean up when you leave Acknowledge the IGB Core as:          “Core Facilities at the Carl R. Woese Institute for Genomic Biology" Let us know ...

IGB Core Instrument LSM 900

IGB Core Training Basic Optics

  405nm, 488nm, 561nm, 640nm excitation. Zen Blue file:///C:/Users/gfried/Downloads/EN_poster_Beampath-LSM-900_A1.pdf